Silver nanoparticles from Hpytus suaveolens and their effect on biochemical and physiological parameter in mesquite plants

Abstract

In this study, a simple and economical procedure to obtain silver nanoparticles using leaf-extract of Hpytus sauveolens (AgNPs) and their effect in chlorophyll, nitrogen balance index and epidermal polyphenols contents, photochemical efficiency and phenylalanine ammonia lyase (PAL) activity in Prosopis juliflora is reported. Synthesized nanoparticles were characterized by ultraviolet (UV) spectroscopy, DLS analyzer, FTIR spectroscopy, Scanning electron microscopy (SEM) and energy dispersive analysis X-ray (EDX). The results showed that AgNPs contained bioactive molecules, which had important function in the reduction of Ag ion. The AgNPs had mean particle size, zeta potential conductivity of 22 nm, 29.50 mv and 1.394 mS/cm, respectively according to DLS analysis. Different doses of AgNPs (100, 75, 50 and 25 ppm) showed positive effects on phenylalanine ammonia lyase (PAL) activity and neutral effect in chlorophyll, nitrogen balance index and epidermal polyphenols contents on plants. Only higher doses (100 ppm) caused negative effects in photochemical efficiency in Prosopis juliflora . Hence future studies are necessary to elucidate the participation of green AgNPs as exogenous inducers of PAL enzyme involved in the phenylpropanoid pathway in Prosopis juliflora in the reduction of stress oxidative in plants. • Synthesis of green silver nanoparticles from native plants using Hpytus suaveolens . • The applications of AgNPs produce changes biochemical in Prosopis juliflora . • Higher doses AgNPs produce negative effects on physiological status. • Green AgNPs increased the PAL activity in plants.