Silver nanoparticles exhibit ecotoxicological effects via oxidative stress, inflammation, and reproductive toxicity in Asian clam (Corbicula fluminea)

Abstract

Silver nanoparticles (AgNPs) are pervasive environmental pollutants capable of inducing toxicological impacts on benthic organisms. In this study, the effects of AgNPs on the antioxidant enzyme activities, tissue damage, inflammatory responses, and reproductive toxicity of Corbicula fluminea were investigated. C. fluminea was exposed to four concentrations of AgNPs (0, 5 mg/L, 10 mg/L, and 125 mg/L) for 48 h. The results showed that the higher concentrations of AgNPs caused severe tissue damage in multiple organs of C. fluminea, induced oxidative stress and an imbalance of the antioxidant enzyme activities (such as SOD, CAT, MDA), and increased the inflammatory immune response involving NFκB, TLR2/4, HSP70/90, IL1β, and TNFα. Notably, further transmission electron microscopy and cytological analyses revealed that AgNPs exposure induced apoptosis in the gonad tissues, resulting in significant loss and damage in the oocytes and spermatids. The present study demonstrates the ecotoxicological impacts of AgNPs on freshwater bivalves, particularly highlighting their reproductive toxicity on germ cells, signifying the potential toxic effects of heavy metal pollution on aquatic ecosystems.