Abstract
This study aimed first to prepare silver nanoparticles AgNPs in a chemical reduction, The silver nanoparticles AgNPs properties were characterized using spectroscopy of Vision and UV- Visible (UV- Vis), and atomic force microscopy (AFM) and a ZetaSizer device. This study also aimed to study the results effect of preparing Silver nanoparticles on the Leishmania parasite using an artificial environment. The results of the spectrophotometer of the secondary silver solution with yellow color, showed maximum absorption at a wavelength of 400 mm, while the image of atomic force microscope (AFM) showed the hierarchical morphology shape of the prepared Silver nanoparticles with an average size of (10- 30 nm). Silver secondary particles were estimated with a diameter ranging from (12- 60 nm) and with good dispersion in water by ZetaSizer device. The results illustrated that the number of Promastigotes of the Leishmania parasite decreased after adding the Silver nanoparticles AgNPs in varying proportions, as the concentration of 500 μg/ml had the highest effect after 72 hours from incubation, and the percentage of remaining parasites was 5.2% comparing with the control percentage of 14.99%, while the value of IC50 was 253.075 μg/ml, The value of R2 = 0.8809, slope = 0.0478 after 24 hours from incubation.
Highlights
Cutaneous leishmania Disease is known by common names (Aleppo pill, eastern pill, Baghdad pill) and it is one of the most common and widespread types of leishmania, and is currently a major public health concern in developing countries
The catalytic properties of the Silver nanoparticles AgNPs: Silver nanoparticles (AgNPs) contribute to the release of silver ions from the crystal surface of the nanomaterial and their ability to remain for a long period of time, which enables them to carry out oxidation processes and ionic reactions that lead to the removal of bacteria and parasites inside cells. [7]
(200- 1100 nm), where the nanoparticles were suspended in distilled water, and through it an absorption peak was observed at 400 nm, which indicates the formation of silver nanoparticles as in Figure (1)
Summary
Cutaneous leishmania Disease is known by common names (Aleppo pill, eastern pill, Baghdad pill) and it is one of the most common and widespread types of leishmania, and is currently a major public health concern in developing countries. Because of the wide application of nanotechnology in the field of biological sciences in recent years, the creation of silver nanoparticles and their use in treating diseases was of great importance due to their distinctive physical, electrical, chemical and biological properties, so nanoparticles have great scientific importance because they have a great ability to improve human life, especially in the medical field. Research in the field of nanotechnology has increased due to the difference in the properties of the material at the nanoscale from its observed properties at natural size due to the difference in the surface factor to the size. Silver nanoparticles have unique properties, such as electrical conductivity and stability, and are widely used in medical fields, nanomedicine, food industries, biological studies and biomedical photography, and it has effective antibacterial and antiparasitic factor. The chemical method that is considered one of the most desirable processes due to its simplicity, versatility of uses, and abundance of raw materials at low cost, which makes it possible to use this method to prepare nanoparticles AgNPs popular because it is characterized by saving time, cost and great effectiveness. [10] In this method, reagents such as formaldehyde, ethylene glycol, sodium citrate, ascorbic acid and sodium borohydride are used as reducing agents, and the formation of silver nanoparticles can be observed by formation different colors according to the nanoscale size of the formed particles. [11]
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