Silver Nanoparticle-Enzyme Composite Films for Hydrogen Peroxide Detection

Abstract

For high-performance biosensor development, two key issues to be significantly considered are the effective integration of biological enzymes with electrode transducer and the rapid communication across the biosensing interface. To combat these issues, the present work reported a robust enzymatic biosensor platform based on binary nanoparticles film composed of enzyme-loaded polymeric nanoparticles and conductive silver nanoparticles (Ag NPs) by a facile electrophoretic deposition method. First, horseradish peroxidase (HRP) as a model enzyme was coassembled with an amphiphilic and photo-cross-linkable polypeptide of 7-amino-4-methylcoumarin chemically modified poly(γ-glutamic acid) (γ-PGA–AMC, PGA-C), generating enzyme-loaded colloidal nanoparticles (HRP@PGA-C NPs) in aqueous solution. Thanks to the presence of abundant carboxyl groups in HRP@PGA-C NPs, they were electrodeposited simultaneously with water-dispersed silver colloidal nanoparticles (Ag NPs) onto the electrode surface, generating a robust bio...