Silver nanoparticle (AgNPs) doped gum acacia–gelatin–silica nanohybrid: An effective support for diastase immobilization

Abstract

An effective carrier matrix for diastase alpha amylase immobilization has been fabricated by gum acacia–gelatin dual templated polymerization of tetramethoxysilane. Silver nanoparticle (AgNp) doping to this hybrid could significantly enhance the shelf life of the impregnated enzyme while retaining its full bio-catalytic activity. The doped nanohybrid has been characterized as a thermally stable porous material which also showed multipeak photoluminescence under UV excitation. The immobilized diastase alpha amylase has been used to optimize the conditions for soluble starch hydrolysis in comparison to the free enzyme. The optimum pH for both immobilized and free enzyme hydrolysis was found to be same (pH=5), indicating that the immobilization made no major change in enzyme conformation. The immobilized enzyme showed good performance in wide temperature range (from 303 to 323K), 323K being the optimum value. The kinetic parameters for the immobilized, (Km=10.30mg/mL, Vmax=4.36μmolmL−1min−1) and free enzyme (Km=8.85mg/mL, Vmax=2.81μmolmL−1min−1) indicated that the immobilization improved the overall stability and catalytic property of the enzyme. The immobilized enzyme remained usable for repeated cycles and did not lose its activity even after 30 days storage at 40°C, while identically synthesized and stored silver undoped hybrid lost its ∼31% activity in 48h. Present study revealed the hybrids to be potentially useful for biomedical and optical applications.