Silver-impregnated platelet-rich fibrin as a barrier membrane

Abstract

Introduction: Platelet-rich fibrin (PRF) was developed to eliminate xenofactors form of platelet-rich plasma to be used as a source of growth factor for tissue regeneration. PRF has been used as an autologous grafting material because of its ability to accelerate physiologic wound healing and new bone formation. Aim: To compare mechanical and histologic characteristics of the PRF membrane and silver-impregnated PRF. Materials and Methods: Venous blood was taken from the subjects for PRF preparation. Then, 2 normal PRFs were prepared, and for silver-impregnated PRF, 9 ml of blood in addition to 1 ml of silver nanoparticle suspension was poured into another tube and gently shook with hand to achieve a uniform 1% concentration. The tubes were centrifuged at 3000 rpm for 10 min. The tensile test was done by the universal testing machine: The remaining pieces of the membranes were fixed in 10% formalin for 24 h to be subjected to hematoxylin and eosin staining and evaluated with the light microscope Degradation time. Results: Silver-impregnated showed improved mechanical properties and dense fibrin network than PRF. Conclusion: Silver-impregnated PRF membrane demonstrated properties to be used as a barrier membrane for periodontal reconstruction.