Abstract
Nanotechnology involves the synthesis of nanoparticles that have been used in the therapeutic application for treating diseases. In this present study, we have adopted the synthesis of myconanoparticles from the extracellular extract of endophytic fungi Penicillium sclerotiorum (PsNps) and validated its antibacterial potential against antibiotic-resistant uropathogenic E. coli and ATCC (25,922) strain of Escherichia coli. Endophytic fungi were isolated from the healthy leaves of Tamarindus indica. The genomic DNA from endophytic fungi was isolated and the ITS region was amplified by polymerase chain reaction (PCR) using universal fungal primers ITS1 and ITS4 and sequenced for the identification of endophytic fungal isolates. Penicillium sclerotiorum extract was used for the synthesis of silver nanoparticles (PsNps) and was characterized by UV-vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), zeta potential, FE-SEM, and Energy dispersive X-ray analysis (EDAX). Antibacterial activity of PsNps was tested against the antibiotic-resistant uropathogenic E. coli and ATCC (25,922) strain of E. coli. Further experiments were carried out to explore the potential of PsNps in regulating the CTX-M-15 gene. The antimicrobial activity showed that the PsNps inhibited growth, biofilm formation in both the strains of E. coli. The expression of the gene encoding CTX-M-15 was downregulated in a resistant strain of uropathogenic E. coli. Our results suggest that the PsNps could be used as an alternative source for antibiotics. Thus, further studies can be conducted to prove the in vivo potential of PsNps and can be formulated for commercialization.
Highlights
Endophytic fungi are the endosymbionts that are present in healthy tissue of plants
In this present study we have adopted the synthesis of myconanoparticles from the extracellular extract of endophytic fungi Penicillium sclerotiorum (PsNps) and validated for its antibacterial potential against antibiotic resistant uropathogenic E. coli and ATCC (25922) strain of E. coli
Antibacterial activity of PsNps was tested against the antibiotic resistant uropathogenic E. coli and ATCC (25922) strain of E. coli
Summary
Endophytic fungi are the endosymbionts that are present in healthy tissue of plants. Endophytic organism plays an important role in higher plants by inducing disease resistance. Endophytes are rich in producing large number of active secondary metabolites which help its host to combat the microbial diseases. Previous literature support that isolated endophytic fungi from medicinal plants showed antifungal, antibacterial, antimicrobial properties against different pathogens [1]. Secondary metabolites have a broad array of applications in various fields especially in pharma industry. Endophytic microorganisms are great source of natural and novel bioactive compounds that can be used to meet pharmaceutical, medical and industrial demand. Under optimum conditions endophytes deliver large number of secondary metabolites which are extremely effective against detrimental pathogens such as MDR microbes [2, 3]
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