Abstract
Phosphophoryn, a higWyphosphorylated protein, is the most abundant protein among the non-collagenous protein of dentine. The staining of phosphophoryn can be done by using the silver colloid staining. In this paper, the staining effect of the silver colloid stain on both non-sclerotic and sclerotic dentine was investigated. Eight teeth from donors aged 14, 17, 22, 34, 55, 57, 60 and 65 were used for this experiment. The younger teeth were used to demonstrate normal root dentine while the older age teeth were used to demonstrate sclerotic root dentine at the apical region of the root. There was no staining of the normal root dentine as compared to sclerotic dentine when the silver colloid staining was used.
Highlights
Organic matrix of dentine is comprised of collagen fibres and a variety of non-collagenous proteins
Dentine phosphophoryn can be found in circumpulpal dentine but not in mantle dentine, reparative dentine and dentine of dentinogenesis imperfecta, a genetic disorder of dentine formation [3]
The sclerotic dentine of the apical root region of the older aged teeth was not stained by the silver colloid
Summary
Organic matrix of dentine is comprised of collagen fibres and a variety of non-collagenous proteins. Dentine phosphophoryn can be found in circumpulpal dentine but not in mantle dentine, reparative dentine and dentine of dentinogenesis imperfecta, a genetic disorder of dentine formation [3]. Such evidence suggests that dentine phosphophoryn is synthesised and secreted only by physiologically differentiated odontoblasts but not by undifferentiated or damaged cells. Silver colloid staining was initially developed by Smith et al [5] for the detection of 'nucleolar organiser regions' (NORS) in histological sections. Whilst examining NORs activity during odontogenesis with this silver colloid stain, they observed specific reactivity with the dentine extracellular matrix in demineralised histological sections. The organic matrix of peritubular dentine was found to have the greatest
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