Abstract
AgNOR proteins are silver-stainable proteins found in the nucleolus, and this silver staining property is used to distinguish certain malignant tumors from benign tumors. Transcription factor UBF (upstream binding factor) and RNA polymerase I large subunit have been shown to be members of this group of nucleolar proteins. This study shows that UBF is 20 times more sensitive to silver staining than RNA polymerase I large subunit; aspartic acid residues, but not glutamate residues, are responsible for silver staining of UBF, and at least 2 adjacent aspartate residues in an acidic region are required for efficient silver staining. (The J Histotechnol 21:13, 1998)
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