SILKWORM PUPAE PROTECT AGAINST ALZHEIMER’S DISEASE

Abstract

Silkworm ( Bombyx mori) pupae have long been used as food and medicine in Asian countries. It is reputed for the treatment of numerous neurological disorders re lated to oxidative stress including stroke. Therefo re, we hypothesized that silkworm pupae could attenuate memory impairment and neurodegeneration in Alzheimer’s disease. In the present study, we determined the ef fect of silkworm pupae on the neurodegeneration and memory impairment in animal model of Alzheimer’s disease. Adult male Wistar rats, weighing 180-220 g, were orally given the silkworm pupae at doses of 60, 90 and 135 mg kg -1 BW 14 days before and 7 days after the bilateral administration of AF64A, a cholinotoxin, via intrac erebroventricular route. The animals were determined the memory using Morris water maze test and determined the density of neurons in hippocampus. All doses of silkworm pupae used in this study significantly mit igated the memory impairment and the decreased neurons density in hippocampus. To explore the possible und erlying mechanism of the cognitive enhancing effect and neuroprotective effect, the activity of acetylcholi nesterase enzyme and the Malondialdehyde (MDA), the oxidative marker were determined respectively. Our results clearly demonstrated that the cognitive enh ancing effect of silkworm pupae occurred at least via the increased cholinergic function while its neuroprote ctive effect occurred via the decrease oxidative stress. In conc lusion, silkworm pupae appear to be the potential f unctional food to protect against Alzheimer’s disease.