Abstract
BackgroundIn this study, silk fibroin (SF) composite hydrogels containing hydroxyapatite (HAP) nanoparticles (NPs) for bone tissue engineering were fabricated using gamma-ray (γ-ray) irradiation treatment. During the irradiation, the HAP dispersed SF solution was changed to the chemically crosslinked SF hydrogel.MethodsDistribution of HAP NPs in the SF hydrogel was examined by SEM imagery and energy dispersive X-ray spectrophotometry, and the crystalline structure of SF composite hydrogels was also confirmed by X-ray diffractometry. An optimum preparation condition of the SF/HAP composite hydrogels was determined with various HAP contents. For evaluation of the osteogenic differentiation of human mesenchymal stem cells (hMSCs), alkaline phosphatase activity (ALP), HAP nucleation in SBF and in vitro calcium accumulation were measured.ResultsThe results revealed that compared with the pure SF hydrogels, the SF/HAP composite hydrogels improved osteogenic differentiation.ConclusionThis paper demonstrates the great potential of the SF/HAP composite hydrogels in terms of the production of the bone tissue engineering scaffolds for which osteogenesis is required.
Highlights
In this study, silk fibroin (SF) composite hydrogels containing hydroxyapatite (HAP) nanoparticles (NPs) for bone tissue engineering were fabricated using gamma-ray (γ-ray) irradiation treatment
HAP NPs were uniformly dispersed on the pore wall of composite hydrogels, and incorporated NPs were increased with increasing HAP concentration
In this study, the SF/HAP composite hydrogels for bone tissue engineering were prepared by gamma-ray irradiation
Summary
Silk fibroin (SF) composite hydrogels containing hydroxyapatite (HAP) nanoparticles (NPs) for bone tissue engineering were fabricated using gamma-ray (γ-ray) irradiation treatment. Scaffolds for bone tissue engineering offer a promising alternative treatment for medical use, as well as a controllable system for studies of biological function, development of biology and pathogenesis [2, 3]. Ceramic scaffolds have excellent stiffness, but are fragile and have low porosity, resulting in loosening of Numerous research efforts have addressed the development of an ideal scaffold for bone tissue engineering [7, 8]; they still have several limitations. Biodegradability, controllable strength, and good oxygen and water permeability, silk fibroin (SF) originated from Bombyx mori has been fabricated for various tissue engineering scaffolds with various chemical, structural and biochemical modifications. Crosslinked SF hydrogels have been produced through the induction of the β-sheet structure in
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