Silk fibroin hydrogel/dexamethasone sodium phosphate loaded chitosan nanoparticles as a potential drug delivery system

Abstract

The application of nanoparticles-loaded hydrogel as a novel formulation has gotten much attention for a potential drug delivery method for desire drug controlling and targeting. This study prepared a sustained release formulation using dexamethasone sodium phosphate-loaded chitosan nanoparticles embedded in silk fibroin hydrogel. Dexamethasone sodium phosphate-loaded chitosan nanoparticles (DEX-CSNPs) was developed using the ionotropic-gelation technique and inserted in the silk fibroin hydrogel (SFH). Mean particle size, polydispersity index (PDI), and zeta potential of DEX-CSNPs were 488.05±38.69 nm, 0.15±0.07, 32.12±2.42 mV, respectively. The encapsulation efficiency (EE), drug loading capacity (LC), and the cumulative amount of released drug of DEX-loaded CSNPs, which detected in phosphate buffer saline (PBS) solution, were 67.6±6.7%, 15.7±5.7%, and 75.84%, respectively. The DEX-CSNPs were then mixed with silk fibroin (SF) solution and induced gelation by sonication to prepare a drug-releasing system. As a result, the scanning electron microscopy (SEM) image shows that the prepared drug delivery system had a properly interconnected porous structure. Smaller pore size, greater porosity, higher water uptake, and swelling ratio were achieved by incorporating CSNPs and DEX-loaded CSNPs. The cytotoxicity study was performed for the L929 fibroblast cell line. The drug release kinetics study was performed on a prepared drug delivery system. Finally, the release test results showed a suitable extended-release of DEX from the carrier over 16 days. Overall, the developed drug-releasing system can be a promising candidate for drug delivery applications.