Silk fibroin electrospun nanofiber blends with antibiotics and polyvinyl alcohol for burn wound healing

Abstract

AbstractSilk fibroin, is a structural and protein‐based biomacromolecule produced from Bombyx mori silkworm. Silk fibroin has been widely being used for biomedical and biomaterial applications, tissue engineering, and drug delivery since a decade. The present study mainly focused to investigate the wound healing efficiency of the developed silk fibroin‐based extracellular electrospun nanofibrous matrix. Electrospun nanofibers were developed by blending the silk fibroin solution with antibiotics (Ciprofloxacin, Amoxicillin, and Nystatin) and polyvinyl alcohol. Results have shown that the developed extracellular matrix has antimicrobial sensitivity activity against the pathogens which causes infection by forming pus at the site of burn wounds. The cell adhesion and cell viability studies have shown a positive effect on L929 fibroblast cell lines. The morphological studies were carried out on SEM. The structural conformations of the developmental matrix were carried out on XRD. The wound healing efficiency of the developed matrix was checked by using a rat model. The results exhibited a dose dependent healing efficiency. The present results demonstrate the developed silk fibroin extracellular matrix promotes cell proliferation and helps in wound healing by controlled release of the antibiotics without losing their chemical properties.