Abstract
Spherical polymeric microbeads were produced by suspension cross-linking of hydroxyl-terminated polydimethylsiloxane prepolymers. Dimethylsiloxane-ethyleneoxide copolymer, dibutyltin dilaurate and methyltriethoxysilane were used as surfactant, catalyst and cross-linker, respectively. The surfaces of the microbeads were further modified by exposure of 3-amino-propyltriethoxysilane plasma in a glow-discharge apparatus. These silicone-based microbeads were used as microcarriers in culturing of baby hamster kidney (BHK) cells both in stationary and in submerged culture conditions. High attachment and growth were achieved on the glow-discharge treated silicone microbeads with moderate surface wettabilities (contact angle: 43−46°).
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