Abstract
BackgroundTissue and organ regeneration via transplantation of cell bodies in-situ has become an interesting strategy in regenerative medicine. Developments of cell carriers to systematically deliver cell bodies in the damage site have fall shorten on effectively meet this purpose due to inappropriate release control. Thus, there is still need of novel substrate to achieve targeted cell delivery with appropriate vehicles. In the present study, silicon based photovoltaic (PV) devices are used as a cell culturing substrate for the expansion of myoblast mouse cell (C2C12 cells) that offers an atmosphere for regular cell growth in vitro. The adherence, viability and proliferation of the cells on the silicon surface were examined by direct cell counting and fluorescence microscopy.ResultsIt was found that on the silicon surface, cells proliferated over 7 days showing normal morphology, and expressed their biological activities. Cell culture on silicon substrate reveals their attachment and proliferation over the surface of the PV device. After first day of culture, cell viability was 88% and cell survival remained above 86% as compared to the seeding day after the seventh day. Furthermore, the DAPI staining revealed that the initially scattered cells were able to eventually build a cellular monolayer on top of the silicon substrate.ConclusionsThis study explored the biological applications of silicon based PV devices, demonstrating its biocompatibility properties and found useful for culture of cells on porous 2-D surface. The incorporation of silicon substrate has been efficaciously revealed as a potential cell carrier or vehicle in cell growth technology, allowing for their use in cell based gene therapy, tissue engineering, and therapeutic angiogenesis.
Highlights
Tissue and organ regeneration via transplantation of cell bodies in-situ has become an interesting strategy in regenerative medicine
We describe the use of a commercially available monocrystaline silicon PV device to be used as substrate for culturing of C2C12 mammalian cells
Materials Silicon substrate preparation Silicon based photovoltaic (PV) devices that convert the energy of sunlight directly into electricity by the photovoltaic effect were used as silicon substrate for cell culturing
Summary
Tissue and organ regeneration via transplantation of cell bodies in-situ has become an interesting strategy in regenerative medicine. Silicon based photovoltaic (PV) devices are used as a cell culturing substrate for the expansion of myoblast mouse cell (C2C12 cells) that offers an atmosphere for regular cell growth in vitro. Bio processed cells in the various forms provide unique potential to customize the cells to damage sites where the cells or tissues are required as therapeutic agent. Laboratory processed cells can be delivered to targeted site of patient [1,2,3]; cell delivery via cell substrate provides mechanical and biological support for attachment and proliferation [4,5] of cells. In our investigation silicon based photovoltaic (PV) devices are used as cell culturing substrates for mammalian myoblast cells, C2C12 Compare to three dimensional (3D) cell structures, thin two dimensional (2D) cell construct does not required complicated microvasculature and are easy to fabricate and handle [6].
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