Silicon nutrition alleviates physiological disorders imposed by salinity in hydroponically grown canola (Brassica napus L.) plants

Abstract

The effects of Si nutrition on transpiration, leaf anatomy, accumulation of Na+, K+, Cl−, P, Fe and B and some reactive oxygen species related parameters were investigated in canola plants under salinity. Plants were grown hydroponically in growth chamber under controlled conditions at 0 and 100 mM NaCl each supplied with or without 1.7 mM silicon (Si) as sodium silicate. Salinity imposed significant reduction in growth parameters of plants like fresh weights of roots and shoots and leaf area. It also led to accumulation of Na+ and Cl− and a decrease in the concentration of K+, P, B and Fe. Reduction of transpiration, stomatal density and specific leaf area in leaves and an increase in leaf thickness were amongst other symptoms in salt-affected plants. Salinity led to higher concentration of hydrogen peroxide, increased lipid peroxidation and decrease of catalase and peroxidase activity, which suggests the induction of oxidative stress in plants. Silicon nutrition could prevent toxic ions (Na+ and Cl−) accumulation while higher levels of essential minerals like K+, P and Fe were maintained in plants. Consequently, silicon nutrition decreased oxidative stress in plants, evidenced by increase in antioxidant enzyme activity, reduction in hydrogen peroxide and lipid peroxidation.