Silicon nanoparticles confer hypoxia tolerance in citrus rootstocks by modulating antioxidant activities and carbohydrate metabolism

Abstract

Citrus is a remarkable fruit crop, extremely sensitive to flooding conditions, which frequently trigger hypoxia stress and cause severe damage to citrus plants. Silicon nanoparticles (SiNPs) are beneficial and have the potential to overcome this problem. Therefore, the present study aimed to investigate the effect of silicon nanoparticles to overcome hypoxia stress through modulating antioxidant enzyme activity and carbohydrate metabolism. Three citrus rootstocks (Carrizo citrange, Roubidoux, and Rich 16–6) were exposed to flooding (with and without oxygen) through different SiNP treatments via foliar and root zone. SiNPs applied treatment plants showed a significant increase in photosynthesis, leaf greenness, antioxidant enzymes, and carbohydrate metabolic activities, besides the higher accumulation of proline and glycine betaine. The rate of lipid peroxidation was drastically higher in flooded plants; however, SiNPs application reduced it significantly, ultimately reducing oxidative damage. Overall, Rich16-6 rootstock showed good performance via root zone application compared to other rootstocks, possibly due to genotypical variation in silicon uptake. Our outcomes demonstrate that SiNPs significantly affect plant growth during hypoxia stress conditions, and their use is an optimal strategy to overcome this issue. This study laid the foundation for future research to use at the commercial level to overcome hypoxia stress and a potential platform for future research.