Abstract
Adding silicon to the culture medium may contribute, to improve many growth parameters including embryogenesis and organogenesis. The objective of this study was to evaluate the embryogenic potential of passion fruit in vitro (Passiflora edulis f. Flavicarpa O. Deg), submitted to different concentrations of silicon. Nodal segments of plants were inoculated at five concentrations of silicic acid added to the culture medium, 0.0; 0.5; 1.0; 1.5 and 2.0 g L-1. After 60 days, the visual characteristics (% contamination, callus, bud, root and oxidation), cytochemical test and microanalysis of X-rays evaluated. Concentrations of 0.5; 1.0 and 2.0 g L-1 of silicon did not differ in relation to callus formation. By the cytochemical test, all treatments presented embryogenic cells indicated by the reaction to acetic Carmine and, little reaction to the Evans blue associated with cell death. By the microanalysis of X-rays, the nutrients sulfur and phosphorus, presented significant absorption responses at the concentration of 1.5 g L-1 of silicon in comparison to the other concentrations. The silicon added to the culture medium has a response in the formation of cells with embryogenic potential in nodal segments of passion fruit. The concentration of 2.0 g L-1 of silicon acid (H4SiO4) has a high embryogenic potential in passionflower cells, Passiflora edulis f. Flavicarpa O. Deg.
Highlights
The passion fruit belongs to the genus Passiflora, which is considered the largest and most economically important of the Passifloraceae family (Pérez et al, 2007; Braglia et al, 2010)
The regeneration was seeing embryogenesis somatic be shown to be promising and it has the potential to devote it self in the field of the micropropagation, which is the way morphogenic principal of the regeneration in the type Passiflora (Zerbini et al, 2008; Pinto et al, 2010), in the improvement of result of passion fruit
Considering the benefits that silicon promotes to plants and due to the lack of studies in passion fruit, especially with regard to in vitro silicon, the objective of the work was to adjust the concentration of silicon in the induction of the embryogenic potential of plant cells in Passiflora edulis f
Summary
The passion fruit belongs to the genus Passiflora, which is considered the largest and most economically important of the Passifloraceae family (Pérez et al, 2007; Braglia et al, 2010). Is an allogeneic plant, has autoincompatibility of the sporophytic type (Ferreira et al, 2010). The propagation of this plant is preferably realized of form sexually, through seed or asexually, by the use of cutting, grafting and tissue culture in vitro (Sousa et al, 2010). The tissue culture through the micropropagation is an important method to multiply quickly the high production and/or disease genótipos resistant superiors in the physical space and reduced time, very often offering the only alternatives for programs of plant breeding (Zerbini et al, 2008; Silva et al, 2013). The regeneration was seeing embryogenesis somatic be shown to be promising and it has the potential to devote it self in the field of the micropropagation, which is the way morphogenic principal of the regeneration in the type Passiflora (Zerbini et al, 2008; Pinto et al, 2010), in the improvement of result of passion fruit
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