Silencing STAT1 gene enhances radiosensitivity of radioresistant human nasopharyngeal carcinoma CNE-2R cells

Abstract

Objective To study the effect of silencing STAT1 on radiation sensitivity of nasopharyngeal carcinoma cells. Methods Lentivirus-mediate STAT1 gene-transfer was performed to radioresistant human nasopharyngeal carcinoma CNE-2R cells and the transfection efficiency was detected by RT-PCR. The cell proliferation was measured by MTT, and the apoptosis was analyzed by flow cytometry. The radiosensitization ratio was measured according to the clone formation efficiency. Results After transfection with STAT1 siRNA, the expression of STAT1 was effectively inhibited in CNE-2R cells(F=429.87, P 0.05). Conclusions Silencing STAT1 could increase radiosensitization of nasopharyngeal carcinoma cells. Key words: STAT1; RNA interference; Lentiviral vector; Radiosensitivity; Nasopharyngeal carcinoma