Abstract
RNA interference (RNAi) using exogenous double-stranded RNA (dsRNA) has been used to silence the model gene of Nicotiana benthamiana phytoendesaturase. Here we report on an efficient technique for dsRNA synthesis using E. coli HT115 strain. This strain is deficient in RNase III, an enzyme that normally destroys most dsRNA in a bacterial cell and has been engineered to produce big quantities of dsRNA. We also used root treatment for dsRNA delivery to N. benthamiana plants. We found this method to be one of the most efficient ways to deliver dsRNA for plants.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: IOP Conference Series: Earth and Environmental Science
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
