Abstract
BackgroundPolycystic ovary syndrome (PCOS) is the most common hormonal disorder among reproductive-aged women worldwide, however, the mechanisms and progression of PCOS still unclear due to its heterogeneous nature. Using the human granulosa-like tumor cell line (KGN) and PCOS mice model, we explored the function of lncRNA UCA1 in the pathological progression of PCOS.ResultsCCK8 assay and Flow cytometry were used to do the cell cycle, apoptosis and proliferation analysis, the results showed that UCA1 knockdown in KGN cells inhibited cell proliferation by blocking cell cycle progression and promoted cell apoptosis. In the in vivo experiment, the ovary of PCOS mice was injected with lentivirus carrying sh-UCA1, the results showed that knockdown of lncRNA UCA1 attenuated the ovary structural damage, increased the number of granular cells, inhibited serum insulin and testosterone release, and reduced the pro-inflammatory cytokine production. Western blot also revealed that UCA1 knockdown in PCOS mice repressed AKT activation, inhibitor experiment demonstrated that suppression of AKT signaling pathway, inhibited the cell proliferation and promoted apoptosis.ConclusionsOur study revealed that, in vitro, UCA1 knockdown influenced the apoptosis and proliferation of KGN cells, in vivo, silencing of UCA1 regulated the ovary structural damage, serum insulin release, pro-inflammatory production, and AKT signaling pathway activation, suggesting lncRNA UCA1 plays an important role in the pathological progression of PCOS.
Highlights
Polycystic ovary syndrome (PCOS) is the most common hormonal disorder among reproductive-aged women worldwide, the mechanisms and progression of PCOS still unclear due to its heterogeneous nature
Silencing of urothelial carcinoma‐associated 1 (UCA1) regulated the proliferation and apoptosis of KGN cells To determine the roles of UCA1 in the proliferation and apoptosis of KGN cells, we transfected sh-UCA1 and shNC into KGN cells to knock down the expression of UCA1
Using CCK8-assay to detect cell proliferation rate for 5 days, the results showed that silencing of UCA1 in KGN cells prominently suppressed the cell proliferation (Fig. 1b)
Summary
Polycystic ovary syndrome (PCOS) is the most common hormonal disorder among reproductive-aged women worldwide, the mechanisms and progression of PCOS still unclear due to its heterogeneous nature. Using the human granulosa-like tumor cell line (KGN) and PCOS mice model, we explored the function of lncRNA UCA1 in the pathological progression of PCOS. Polycystic ovary syndrome (PCOS) is a common hormonal disorder among reproductive-aged women worldwide. The study of PCOS began in 1953, the mechanisms and pathogenesis of PCOS remain unclear due to its heterogeneous nature [5]. KGN is a type of human granulosa like tumor cell line, which maintains most of the normal GCs physiological characteristics, due to its good growing ability in culture, KGN cells are normally applied to the study of the molecular mechanism and function of GCs [9]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
