Silencing of Cytochrome P450 Gene AgoCYP6CY19 Reduces the Tolerance to Host Plant in Cotton- and Cucumber-Specialized Aphids, Aphis gossypii.

Abstract

Cytochrome P450 monooxygenases play important roles in insect metabolism and detoxification of toxic plant substances. However, the function of CYP6 family genes in degrading plant toxicants in Aphis gossypii has yet to be elucidated. In this study, AgoCYP6CY19, an A. gossypii CYP gene that differentially expresses in cotton- and cucumber-specialized aphids, was characterized. Spatiotemporal expression profiling revealed that AgoCYP6CY19 expression was higher in second instar nymph and 7 day old adults than in other developmental stages. Although the expression of AgoCYP6CY19 was significantly higher in cotton-specialized aphids, AgoCYP6CY19 silencing significantly increased larval and adult mortality and reduced total fecundity in both cotton- and cucumber-specialized aphids. What is more, the expression of AgoCYP6CY19 was significantly induced after the cotton-specialized and cucumber-specialized aphids fed on epigallocatechin gallate (EGCG) and cucurbitacin B (CucB), respectively. These findings demonstrate that AgoCYP6CY19 plays a pivotal role in toxic plant substance detoxification and metabolism. Functional knowledge about plant toxicity tolerance genes in this major pest can provide new insights into insect detoxification of toxic plant substances and insecticides and offer new targets for agricultural pest control strategies.