Abstract
Bcl2-associated athanogene 3 (BAG3) has been reported to be involved in aggressive progression of many tumors. In the present study, we examined the expression of BAG3 in human cervical cancer (CC) tissues and investigated the role of BAG3 in SiHa and HeLa cell growth, migration, and invasion. Here, we found that most of CC tissues highly expressed the protein and mRNA of BAG3, while their expression was obviously lower in paired normal tissues (all p<0.001). BAG3 expression was associated with FIGO stage and metastasis (all p<0.05). In-vitro analysis demonstrated that BAG3 siRNAs inhibited SiHa and HeLa cell growth, invasion and migration. Mechanically, BAG3 siRNAs inhibited the expression of EMT-regulating markers, involving MMP2, Slug and N-cadherin, and increased the expression of E-cadherin. In a xenograft nude model, BAG3 siRNAs inhibited tumor growth and the expression of EMT biomarkers. In conclusion, BAG3 is involved in the EMT process, including cell growth, invasion and migration in the development of CC. Thus, BAG3 target might be recommended as a novel therapeutic approach.
Highlights
To data, cervical cancer has been reported to be the second most common cancers in the department of gynecology [1]
We found that Bcl2-associated athanogene 3 (BAG3) mRNA and protein were highly expressed in HeLa and SiHa cell lines than Normal human endocervical epithelial cell (NEEC) cells (Figure 1c, 1d)
These results indicate that BAG3 was involved into the development of human cervical cancer
Summary
Most of cervical cancer patients, especially patients with International Federation of Gynecology and Obstetrics (FIGO) IB2 stage, will develop the recurrence and distant metastasis [2]. It has been reported that BAG3 is highly expressed in many kinds of primary tumors, including ovarian cancer, and glioblastoma [10,11,12,13]. Some studies have reported that the overexpression of BAG3 inhibits drug-induced or serum-deprivation apoptosis in some degree and the inhibition of BAG3 promotes tumor cell apoptosis. We examined the expression of BAG3 in human cervical cancer tissues and investigated the role of BAG3 in SiHa and HeLa cell growth, www.impactjournals.com/oncotarget migration, and invasion. This study provides evidence that BAG3 can increase the EMT nature in cervical cancer cells
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
