Abstract
Germin-like proteins (GLPs) are encoded by a family of genes found in all plants, and in terms of function, the GLPs are implicated in the response of plants to biotic and abiotic stresses. CchGLP is a gene encoding a GLP identified in a geminivirus-resistant Capsicum chinense Jacq accession named BG-3821, and it is important in geminivirus resistance when transferred to susceptible tobacco in transgenic experiments. To characterize the role of this GLP in geminivirus resistance in the original accession from which this gene was identified, this work aimed at demonstrating the possible role of CchGLP in resistance to geminiviruses in Capsicum chinense Jacq. BG-3821. Virus-induced gene silencing studies using a geminiviral vector based in PHYVV component A, displaying that silencing of CchGLP in accession BG-3821, increased susceptibility to geminivirus single and mixed infections. These results suggested that CchGLP is an important factor for geminivirus resistance in C. chinense BG-3821 accession.
Highlights
Geminiviridae is a plant virus family whose members infect important crops worldwide
Data were subjected to analysis of variance by the general linear models (GLM) procedure, means comparison by Tukey’s test according to SAS methods [30]
The aforementioned results suggested that the Germin-like proteins (GLPs) protein encoded by CchGLP of C. chinense BG-3821 could be actively participating in the defense mechanism of these plants due to in non-silenced plants inoculated with the empty vector (PHYVV (-C) + wt component B), resistance was displayed to simple and mixed infection with geminiviruses, and this phenotype became susceptible when silencing CchGLP
Summary
Geminiviridae is a plant virus family whose members infect important crops worldwide These viruses contain single-stranded DNA genomes packed in twinned particles [1]. RNA viruses induce a transient silencing that might result in the removal of the viral genome itself or in important differences in transcripts levels [26] In this sense, a Pepper huasteco yellow vein virus (PHYVV-A)-derived vector, has been evaluated for silencing genes Comt, pAmt, and Kas in pepper plants which are involved in the biosynthesis of capsaicinoids. A Pepper huasteco yellow vein virus (PHYVV-A)-derived vector, has been evaluated for silencing genes Comt, pAmt, and Kas in pepper plants which are involved in the biosynthesis of capsaicinoids This approach was successful in silencing the aforementioned genes and leading to a dramatic reduction in capsaicinoid content [27]. The aim of this work was to evaluate the role of CchGLP in natural resistance to geminivirus in Capsicum chinense BG-3821 through silencing the gene using PHYVV-A derived vector
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