Silencing hsa_circ_0007841 Inhibits Cell Proliferation and Promotes Cell Apoptosis via Regulating miR-507 in Multiple Myeloma Cells

Abstract

Recently study has indicated that hsa_circ_0007841 is up-regulated in patients with multiple myeloma and may act as an important biomarker in Multiple myeloma. However, the mechanisms and effects of hsa_circ_0007841 remain unclear and were firstly investigated herein. The gene expression level was detected via PCR assay. The CCK-8 assay was performed to measure the cell viability. The cell proliferation capacity was evaluated via colony formation assay. The protein express level was detected by western blot and cell apoptosis via flow cytometry. The target of hsa_circ_0007841 was predicted via CircInteractome online tool and validated by luciferase reporter assay. Hsa_circ_0007841 was overexpressed and miR-507 was poorly expressed in multiple myeloma cells. Silencing hsa_circ_0007841 has anti-proliferation and pro-apoptosis effects in multiple myeloma cells. MiR-507 was found to be the target of hsa_circ_0007841. Inhibition of miR-507 relieved the effects of silencing hsa_circ_0007841 in myeloma cells. Silencing hsa_circ_0007841 suppressed cellular proliferative ability and enhanced cell apoptosis rate via targeting and up-regulating miRNA-507 in multiple myeloma cells.