Silane treatment of coated carbonate apatite scaffold affects bioactivity and cell viability

Abstract

The aim of this study is to determine effects of surface treatment on coated-carbonate apatite (CO3Ap) scaffold using silane coupling agent of 3-(trimethoxysilyl) propyl methacrylate. The effect of coating properties with and without surface treatment were investigated through in vitro bioactivity and biocompatibility. Firstly, CO3Ap scaffold was fabricated by transforming β-TCP scaffold via hydrothermal treatment. CO3Ap scaffolds were treated in 2% silane solution before coating in chitosan solution (0.5%, 1% and 2%) by dipping method. The coated scaffold without silane treatment denoted as UTR while with silane treatment denoted as TR. It was found that, the presence of Si-OH group enhanced apatite growth on the coated scaffold. TR0.5 has capability to induce apatite growth for 7 days immersion in HBSS compared to UTR0.5. Rougher surface and different surface charge properties have influenced cell viability on the coated CO3Ap scaffold. To be concluded, it is important to note that bioactivity and cell activity were forced by surface energy or topography, composition and electrostatic interaction.