Abstract
The aim of the current study was to determine the ability of the Lactobacillus buchneri M B/00077 strain to degrade xylan, its impact on the quality of silage made from the lignocellulosic biomass of Spartina pectinata L., as well as the efficiency of biogas production. In the model in vitro conditions the L. buchneri M B/00077 strain was able to grow in a medium using xylan as the sole source of carbon, and xylanolytic activity was detected in the post-culture medium. In the L. buchneri M B/00077 genome, genes encoding endo-1,4-xylanase and β-xylosidase were identified. The silages prepared using L. buchneri M B/00077 were characterized by a higher concentration of acetic and propionic acids compared to the controls or the silages prepared with the addition of commercial xylanase. The addition of bacteria increased the efficiency of biogas production. From the silages treated with L. buchneri M B/00077, 10% and 20% more biogas was obtained than from the controls and the silages treated with commercial xylanase, respectively. The results of the current study indicated the strain L. buchneri M B/00077 as being a promising candidate for further application in the field of pretreatment of lignocellulosic biomass.
Highlights
Lignocellulose biomass such as perennial grasses, including species characterized by a C4 photosynthetic pathway e.g. Spartina pectinata L., as well as various agricultural residues and other herbaceous plants, could become an important feedstock for the production of biofuels[1]
The removal of xylan, except for facilitating the hydrolysis of cellulose; xylo-oligomers can facilitate a decrease in enzyme inhibition and this leads to fewer inhibitors in the fermentation process[11]
Another study revealed that an obligate heterofermentative species—Lactobacillus buchneri CD034—which is closely related to L. brevis, has the ability to degrade xylan owing to several genes encoding endo-1,4-β-xylanase, β-xylosidase, and α-l-arabinofuranosidase[24]
Summary
Lignocellulose biomass such as perennial grasses, including species characterized by a C4 photosynthetic pathway e.g. Spartina pectinata L., as well as various agricultural residues and other herbaceous plants, could become an important feedstock for the production of biofuels[1]. Taking into account the abovementioned consideration, the objective of the current work was to investigate the ability of a new Lactobacillus buchneri M B/00077 strain to degrade xylan during fermentation of lignocellulosic biomass of cordgrass (Spartina pectinata L.), its impact on overall silage quality as well as on the specific methane yield and biogas production. For this purpose, the metabolic capability of Lactobacillus buchneri M B/00077 was investigated in model in vitro conditions, and the activity of xylanolytic extracellular enzymes released by the bacteria into the medium was evaluated. To assess the effect of used Lactobacillus buchneri M B/00077 on the hemicellulose content and biogas yield comparative tests with a commercial enzyme (xylanase) were carried out
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
