Abstract
BackgroundSijunziDecoction (SJZD) is a traditional Chinese medicine prescription used to treat the diseases of gastrointestinal tract since ancient times. The objective of this study was to investigate the protective effects of SJZD on TNBS-induced colitis in rats and TNBS-damaged Caco2 cells.MethodsThe rat colitis model was induced by 2, 4, 6-trinitrobenzene sulfonic acid (TNBS). SJZD (2.8 5.6, 11.2 g/kg) or salazosulfapyridine (SASP) (0.4 g/kg) was administrated orally in rats for 7 days. DAI, pathological scores and the expression of claudin-2 were evaluated. Then we explored the effect and mechanism of SijunziDecoction Serum (SJZDS) onTNBS-damaged Caco2 cells to figure out intestinal barrier protective effect and mechanism of SJZD.ResultsSJZD significantly ameliorated the severity of TNBS-induced colitis and downregulated the level of claudin-2 in colonic tissues. SJZDS promoted proliferation and inhibited apoptosis ofTNBS-damaged Caco2 cells. In Caco2 cell monolayers, we provided mechanistic evidence that SJZDS-induced increased TEER and decreased permeability after TNBS damage, which were mediated through claudin-2 and NF-κB pathway, including the upregulation of claudin-2, decreased activity of NF-κB p65, reduced level of NF-κB p65 and MLCK.ConclusionsOur results indicated that SJZD possesses protective effect of intestinal barrier towards TNBS-induced colitis in rats and TNBS-damaged Caco2 cells in vitro. SJZDis a potential protective agent of intestinal barrier that deserves further investigation.
Highlights
SijunziDecoction (SJZD) is a traditional Chinese medicine prescription used to treat the diseases of gastrointestinal tract since ancient times
SJZD decreased histological changes in rats with TNBSinduced colitis From Fig. 1b and c,histological analysis of the rat colonic tissues revealed a significant reduction in colon inflammation and epithelial cells disruption after the administration of SIZD, compared to the trinitrobenzene sulfonic acid (TNBS) group
Erosion of mucosal layers were present in the colon of the TNBS group, and a significantly reduced influx of inflammatory cells and intact architecture of the crypts were observed in the colon of the SJZD and SASP treated rats
Summary
SijunziDecoction (SJZD) is a traditional Chinese medicine prescription used to treat the diseases of gastrointestinal tract since ancient times. The objective of this study was to investigate the protective effects of SJZD on TNBS-induced colitis in rats and TNBS-damaged Caco cells. Inflammatory bowel diseases (IBDs) are chronic recurrent inflammatory diseases of the gastrointestinal tract, mainly including Crohn’s disease (CD) and ulcerative colitis (UC) [1, 2]. The development of IBDs is linked to characteristic changes in the colon, such as epithelial cell necrosis and ulceration, local infiltration by immune cells, including a significant number of neutrophils and. The tight junction complexes play a decisive role in the maintenance of barrier integrity [6]. The claudin protein family is the most important transmembrane proteins, which can impact the tight junction permeability.
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