Abstract
We reported recently that a pituitary-specific transcription factor PROP1 is present in SOX2-positive cells and disappears at the early stage of the transition from progenitor cell to committed cell during the embryonic development of the rat pituitary. In the present study, we examined the localisation and identification of SOX2-positive and PROP1/SOX2-positive cells in the neonatal and postnatal rat pituitaries by immunohistochemistry. Quantitative analysis of immunoreactive cells demonstrated that SOX2-positive pituitary stem/progenitor cells are not only predominantly localised in the marginal cell layer, but also are scattered in the parenchyma of the adult anterior lobe. In the marginal cell layer, the number of PROP1/SOX2-positive cells significantly decreased after postnatal day 15, indicating that a significant quantitative transition is triggered in the marginal cell layer during the first postnatal growth wave of the anterior pituitary. By contrast, other phenotypes of SOX2-positive stem/progenitor cells that express S100β appeared in the postnatal anterior pituitary. These data suggested that quantitative and qualitative transition occurs by acquisition of a novel mechanism in terminal differentiation in the postnatal development of the anterior pituitary.
Highlights
Significant Quantitative and Qualitative Transition in Pituitary Stem ⁄ Progenitor Cells Occurs during the Postnatal Development of the Rat Anterior Pituitary
We previously reported that immunoreactive signal of PROP1 occupies all of the cells in the primordial pituitary Rathke’s pouch at E13.5, localises in the cells migrating to the expanding anterior lobe at E16.5 [14]
In the sexually mature pituitary at P60, PROP1-signals were rarely present in the marginal cell layer, a small number of signals were still observed in the parenchyma of the anterior lobe
Summary
Significant Quantitative and Qualitative Transition in Pituitary Stem ⁄ Progenitor Cells Occurs during the Postnatal Development of the Rat Anterior Pituitary. We reported recently that a pituitary-specific transcription factor PROP1 is present in SOX2-positive cells and disappears at the early stage of the transition from progenitor cell to committed cell during the embryonic development of the rat pituitary. Other phenotypes of SOX2-positive stem ⁄ progenitor cells that express S100b appeared in the postnatal anterior pituitary These data suggested that quantitative and qualitative transition occurs by acquisition of a novel mechanism in terminal differentiation in the postnatal development of the anterior pituitary. One of the non-endocrine cells identified so far comprises a type of folliculo-stellate cell, which was first distinguished by electron microscopy [2] and named in light of its characteristic shape and follicle formation capacity [3] It was defined later by the presence of S100 protein, its functions in the pituitary and embryonic origin remain obscure [4,5]. We presented immunohistochemical observations that a pituitary-specific factor PROP1 consistently coexists with SOX2 throughout the embryonic development of the pituitary [14]
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