Abstract
Focused infrared lasers are widely used for micromanipulation and visualization ofbiological specimens. An inherent practical problem is that off-the-shelf commercialmicroscope objectives are designed for use with visible and not infrared wavelengths. Lessaberration is introduced by water immersion objectives than by oil immersion ones,however, even water immersion objectives induce significant aberration. We present asimple method to reduce the spherical aberration induced by water immersionobjectives, namely by tuning the correction collar of the objective to a value that is ∼ 10% lower than the physical thickness of the coverslip. This results in marked improvementsin optical trapping strengths of up to 100% laterally and 600% axially from astandard microscope objective designed for use in the visible range. The results aregenerally valid for any water immersion objective with any numerical aperture.
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