Abstract
Objective: This study aimed to investigate the expression and biological functions of TNK2 and miR-125a-3p in colon cancer.Materials and methods: The expression of TNK2 and miR-125a-3p in colon cancer tissues was analyzed using data deposited on public databases including UALCAN and ONCOMINE. We verified their expression in colon cancer cell lines by RT-qPCR and western blotting. By regulating the expression of TNK2 and miR-125a-3p in colon cancer cells, their functions and potential mechanisms were explored.Results:TNK2 was overexpressed in colon cancer cell lines, and it was found to directly bind to miR-125a-3p, which was downregulated in these cell lines. Their expression affected the proliferation and invasion of colon cancer cells. Additionally, colon cancer patients with lower TNK2 expression had better prognoses than those with higher TNK2 expression.Conclusion: Our results indicated that TNK2 and miR-125a-3p play critical roles in colon cancer, and could also serve as biomarkers for the diagnosis and prognosis of this malignant disease.
Highlights
Colon adenocarcinoma (COAD) is a malignancy with high morbidity and mortality
TNK2 expression across 24 cancer types in The Cancer Genome Atlas (TCGA) data was analyzed by UALCAN, independent-samples T test was used to analyze the significant differences between different cancers and their adjacent normal tissues, and the result shows that TNK2 expression was universally higher in most types of cancers, including breast cancer, COAD, and pancreatic cancer (Figure 1E)
We further evaluated the expression of TNK2 in colon cancer and paired normal tissues at different tumor stages
Summary
Colon adenocarcinoma (COAD) is a malignancy with high morbidity and mortality. It is the second most prevalent cancer in men and the third in women (Miller et al, 2019). COAD incidence is higher in men and is influenced by age, with a median age of approximately 70 years at the time of diagnosis in developed countries (Chung, 2018). The prognosis of colon cancer is strongly associated with the therapeutic methods employed, which are based on the stage of the disease at diagnosis. Diagnosis at earlier stages indicates better therapy methods and lower mortality. Patients are often diagnosed at an advanced stage due to the lack of effective methods for early detection, which leads to poor prognoses (Favoriti et al, 2016). It is critical to explore the underlying molecular mechanisms involved and to develop more efficient means of diagnosing colon cancer
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