Significant antimicrobial-producing vegetation uniquely shapes the stormwater biofilter microbiome with implications for enhanced faecal pathogen inactivation

Abstract

Biofilters demonstrate promising yet inconsistent removal of faecal pathogens from stormwater. Antimicrobial-producing plants represent safe, inexpensive biofilter design features which can significantly enhance faecal microbe treatment. The microbiota naturally inhabiting biofilters have additionally been established as key mediators of faecal microbe inactivation. To date, however, it remains unknown: (1) to what extent plants, including significant antimicrobial-producing plants, influence the biofilter microbiome; and (2) how this in turn impacts faecal microorganism survival/die-off. The present study employed 16S rRNA sequencing to examine these relationships throughout the soil profiles of differently vegetated biofilters over time. It was found that plants had subtle but significant influences on the composition and structure of resident biofilter bacterial communities, with varying impacts observed throughout biofilter profiles. Bacterial communities inhabiting biofilters comprising significant antimicrobial-producing plants demonstrated distinct compositional and taxonomic differences relative to other configurations. In particular, compared to other biofilters, the best-performing configuration for faecal bacterial treatment, Melaleuca linariifolia (significant antimicrobial-producing plant), exhibited both higher and lower relative frequencies of putative faecal bacterial antagonists (e.g. Actinobacteria) and mutualists (e.g. certain Gammaproteobacteria), respectively. These preliminary findings suggest that antimicrobial plants may enhance populations of microbiota which suppress faecal bacterial survival, and highlight the plant-microbiome relationship as a novel area of focus for optimising biofilter performance.