Significances and Expressions of Serum Nitric Oxide and Bax in Renal Tubular Epithelia in Rats With Renal Ischemia Reperfusion

Abstract

Objective To investigate the significance and expression of serum nitric oxide (NO) and Bax in renal tubular epithelia in rats with renal ischemia reperfusion (IR). Methods Forty-eight male Sprague-Dawley rats were randomly divided into IR group (n=40, further divided into IR 2 h group, IR 6 h group, IR 12 h group, IR 24 h group, IR 72 h group) and control group(n=8). Every group consisted of 8 rats. The rats of renal IR injury exposed to 45 min bilateral renal pedicle clamping. Rats in control group were exposed and their renal pedicles were not clamped. The change tendency of serum NO and Bax in renal tubular epithelia at IR 2 h, IR 6 h, IR 12 h, IR 24 h and IR 72 h were observed. Results The level of serum NO of rats in control group [(73.980±8.356) μmol/L] was lower than that in IR group, and the level of serum NO of rats in IR 12 h group was highest [(137.864±22.317) μmol/L], after that, the levels of serum NO were gradually decreased, and the level of serum NO of rats in IR 72 h group [(85.071±11.204) μmol/L] still did not reduce to normal level. The levels of serum creatinine (Scr) of rats in IR groups were obviously increased as compared with that in control group [(22.775±6.508) μmol/L] than that of IR group. The level of Scr of rats in IR 12 h group was the highest [(120.850±22.237) μmol/L]. The levels of Bax and apoptosis in renal tubular epithelia in rats in control group were lower [2.450±0.639, 0.900±0.385]. After IR 2 h, those were gradually increased, and the levels of Bax and apoptosis in renal tubular epithelia in IR 12 h group were the highest [69.025±6.550, 26.850±1.476]. The pathological lesions of nephridial tissue were most severe at IR 12 h. Conclusions The expression of serum NO and Bax pro-apoptotic protein in renal tubular epithelia were unregulated after IR, and that induced apoptosis. Key words: kidney; ischemia reperfusion; nitric oxide; Bax; apoptosis