Significance of the expression of proliferation-associated nucleolar antigen p120 in human colorectal tumors

Abstract

Nucleolar protein p120 is considered to be associated with cell proliferation and has also been detected in a broad range of human malignant cells and tissues, but not in either normal resting tissue or most benign tumors. To clarify the significance of the expression of p120 in colorectal tumors or to evaluate the contribution of p120 in the development of colorectal carcinoma, the authors developed a monoclonal antibody against p120 and then examined its expression in adenoma, carcinoma, and normal mucosa. In adenomas, p120 expression was shown in none of 13 cases of mild dysplasia (0%), 2 of 15 of moderate dysplasia (13.3%), and in 2 cases of severe dysplasia (100%). p120-positive adenomas of moderate dysplasia tended to be larger and had higher Ki-67 indexes than the negative ones (adenomas of moderate dysplasia). All 27 carcinomas were positive for p120. p120 immunostaining was found in the nuclei and corresponded closely to the prominent nucleoli of tumor cells. In contrast, either weak or the occasional expression of p120 was traced in only one of the nine normal mucosae (11.1%). Three of the transitional mucosae of the carcinoma were also positive for p120. The percentage of p120-positive tumor cells (p120 index) ranged from 3.2% to 86.6%, and the mean p120 indexes of the four adenomas and all carcinomas were 21.3% and 41.5%, respectively. The p120 index was significantly related to the Ki-67 index ( P < .001) in the p120-positive tumors, whereas the p120 index of the carcinoma did not significantly correlate to the known prognostic markers, such as tumor size, stage, or the degree of differentiation. These results thus suggest that the expression of p120 serves as a marker for cells with a high proliferative potential and is linked to the late events of colorectal tumor progression.