Abstract
Based on structural similarity, the yggB gene product of Corynebacterium glutamicum belongs to the family of MscS-type mechanosensitive channels. In order to clarify its physiological significance in response to osmotic shifts in detail, we studied the properties of YggB using both patch-clamp techniques and betaine efflux kinetics. After heterologous expression in an E. coli strain devoid of mechanosensitive channels, in patch-clamp analysis of giant E. coli spheroplasts YggB showed the typical pressure dependent gating behavior of a stretch-activated channel with a current/voltage dependence indicating a strongly rectifying behavior.
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