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Abstract
The effect of estradiol on ATPase activity in the rat uterine subcellular fractions and of phospholipid on ATPase activity in the mitochondrial fraction is reported in this paper. Phospholipids used in this investigation were prepared from female rat liver according to the method of Hanahan et al.(1957). 1. The ATPase activity in the mitochondrial fraction was increased by estradiol administration but nuclear and microsomal ATPase activity was not affected by hormone treatment. 2. Phospholipase C treatment of the rat uterine mitochondrial fraction decreased ATPase activity in this fraction. 3. The addition of phospholipid into both intact and phospholipase-treated groups increased ATPase activity in the mitochondrial fraction. These data suggest that phospholipid may be taking part in the increase of ATPase activity after estrogen administration.
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