Abstract
Abstract Biochemical characterization of the partially purified β-1,4-D-xylanohydrolase from Geobacillus stearothermophilus KIBGE-IB29 revealed its higher catalytic activity and stability in the presence of industrially used metal ions, surfactants and organic solvents. The β-1,4-D-xylanohydrolase displayed 1.85, 1.28 and 1.14 fold increase in its catalytic activity when incubated with K+, Na+ and Cs+ ions, respectively for 60.0 min. The enzymatic activity enhanced up to 171% and 242% when 1.0 mM concentration of Ca2+ and Mg2+ ions was used respectively. An increase in concentration up to 5.0 mM stimulated the β-1,4-D-xylanohydrolase activity up to 3.6 fold in case of ethanol and 1.95 fold with methanol. Formaldehyde, chloroform, Tween 80 and Triton X-100 drastically enhanced the β-1,4-D-xylanohydrolase activity. Furthermore, 2.9 fold increase in activity was noticed with 1.0 mM SDS whereas, EDTA completely inhibited the enzymatic activity. Such an excellent characteristics of β-1,4-D-xylanohydrolase indicate its broad potential to be used in paper and textile industries.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
