Significance of endothelial glycocalyx in fluid overload therapy

Abstract

Objective To observe the changes and significance of endothelial glycocalyx during fluid overload therapy. Methods Fifty-seven patients with fluid overload were enrolled. Arterial blood and urine were collected at 0, 12, 24, 48 and 72 h after enrollment. Enzyme linked immunosorbent assay (ELISA) was used to detect the components of glycocayx, namely hyaluronic acid (HA), Syndecance-1 (Syn-1), acetamide heparin sulfate (HS), and chondroitin sulfate (CS). Results The primary outcome turned out that as compared with the baseline, the levels of HA and Syn-1 were respectively (1.6±0.5)vs. (4.6±0.8) μg/ml, (169.1±44.4)vs. (369.8±79.2) μg/ml, P 0.05. In the group of 48 h, the clearance capacity was the largest, and the decrease of HA and Syn-1 was the most significant, P 0.05). Conclusion Fluid overload can increase the levels of endothelial glycocalyx, and excessive liquid clearance can decrease the levels of endothelial glycocalyx and improve the microcirculation. Key words: Endothelial glycocalyx; Fluid overload; Microcirculation