Significance of Detection of Immunoglobulin M Combined With Myeloperoxidase in Early Diagnosis of Neonatal Bacterial Infection

Abstract

Objective To evaluate the early diagnosis and clinical significance of detection of immunoglobulin M(IgM) combinded with myeloperoxidase(MPO) in neonates with bacterial infection. Methods From July 2007 to July 2008, 25 full-term neonates who were confirmed by bacterial detection of blood, urine, cerebrospinal fluid, and the culture of umbilical secretions were randomly selected into infected group. The other 25 full-term neonates without bacterial infection were chosen as control group. Both groups detected the level of immunoglobulin M and myeloperoxidase by nephelometry and colorimetry. Informed consent was obtained from all participants. Results The concentrations of immunoglobulin M in serum and myeloperoxidase in pre-therapy of infected group and control group were (0.51±0.14) g/L and (0.57±0.11)△A/min, (0.24±0.14)g/L and (0.17±0.08)△A/min, respectively. There had significant difference between the pre-therapy of infected group and control group (P<0.05). However, the concentration of immunoglobulin M in serum increased to (0.64±0.13)g/L and myeloperoxidase reduced to (0.25±0.09)△A/min in second sampling in post-therapy of infected group, the significant difference was found in the concentration of immunoglobulin M in serum and myeloperoxidase between before and after the treatment in infected group (P<0.05). Conclusion The detection of concentration of immunoglobulin M in serum and myeloperoxidase are valuable for early diagnosis of bacterial infection in newborns. Key words: immunoglobulin M(IgM); myeloperoxidase (MPO); bacterial infection; neonate