Significance and quantitative expression of P-glycoprotein, glutathione-S-transferase-π and lung resistance protein in the orbital lymphoma tissue microarray

Abstract

Objective To explore the recurrence and poor prognosis mechanism from the perspective multi-drug resistance (MDR) by detecting the expression of P-gp,GST-π and LRP in the orbital lymphoma.Methods Fifty orbital lymphoma tissue which were diagnosed by pathology after surgery were collected from Tianjin eye hospital and Tianjin first centre hospital.Among them,46 cases were the observation group.Meanwhile,17 reactive lymphoid hyperplasia tissues were collected as contrast group.Orbital lymphoma tissue microarray and immunohistochemical staining was used to detect the expression of P-gp,GST-π and LRP in orbital lymphoma and reactive lymphoid hyperplasia.Results The P-gp expression rates were 87.0％(40/46) and 35.3％(6/17) in the two tumors tissue microarray and there was significant difference between them (x2=16.81,P =0.000).The GST-π expression rates were 76.1％(35/46) and 41.2％(7/17) in the two tumors tissue microarray and there was significant difference between them (x2=6.808,P =0.009).In poor prognosis group and good prognosis group of the orbital lymphoma,the P-gp expression rates were 85.7％(12/14) and 42.9％(9/21) and there was significant difference between them (Fisher test,P =0.033).The GST-π expression rates were 78.6％(11/14) and 38.1％ (8/21) in the two groups and there was significant difference between them (Fisher test,P=0.013).Conclusions By orbital lymphoma tissue microarray,the MDR gene expression can be detected rapidly,it is efficiently and economically.The MDR of orbital lymphoma is associated with the big expression of P-gp and GST-π. Key words: Orbital lymphoma; P-gp; GST-π; LRP; Tissue microarray; Immunohistochemistry