Abstract
Signature-tagged mutagenesis (STM) is a genome-wide functional screening assay based upon an insertional mutation technique to identify mutants having a particular phenotype from a mixed population. The selection phenotype is either negative or positive for identification of mutations in genes which will impact the host as having a diminished or enhanced adaptation to a defined environment. STM has subsequently been used in different biological systems and adapted to use different mutagens, signature tags, and detection methods. Here, STM has been modified and used for genome-wide screening of Pseudomonas aeruginosa to identify genes and their products essential during the infection process or in enhancing virulence in vivo.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
