Abstract
Post-transcriptional control of mRNA is a key event in the regulation of gene expression. From yeast to human cells, P-bodies are cytoplasmic RNA-protein aggregates that play an essential role in this process, particularly under stress conditions. In this work, we show that in the model yeast Saccharomyces cerevisiae cell wall stress induces the formation of these structures. This effect is dependent on multiple elements in the Cell Wall Integrity (CWI) MAPK signalling pathway, a signal transduction cascade responsible for the maintenance of cell integrity under adverse environmental conditions. Remarkably, P-body assembly requires the catalytic activity of the MAPK of the pathway, Slt2/Mpk1. In accordance with the control exerted by this signalling pathway, the timing of P-body formation is similar to that of the activation of the CWI pathway. Noticeably, mRNAs whose expression is regulated by this pathway localize in P-bodies after the cell is exposed to stress following a temporal pattern coincident with CWI pathway activation. Moreover, when these mRNAs are overexpressed in a mutant background unable to form visible P-bodies, the cells show hypersensitivity to agents that interfere with cell wall integrity, supporting that they play a role in the mRNA lifecycle under stress conditions.
Highlights
Post-transcriptional control of mRNA is a key event in the regulation of gene expression
Wild-type cells were transformed individually with plasmids bearing these reporters and grown for one hour in the presence or absence of two compounds that interfere with cell wall integrity through different mechanisms of action
It is important to note that in the microscopy images showing Congo red (CR) treated cells (Figs 1–5), many cells show some fluorescence signal at the mother-bud neck. This signal exhibits a localization pattern different to that corresponding to P-bodies and it www.nature.com/scientificreports mCherry or Pab1-GFP were treated with CR and ZY as described in a, and with 15% ethanol for 30 minutes before being observed by fluorescence microscopy
Summary
Received: 8 October 2018 Accepted: 11 February 2019 Published online: 28 February 2019 stress. We show that in the model yeast Saccharomyces cerevisiae cell wall stress induces the formation of these structures This effect is dependent on multiple elements in the Cell Wall Integrity (CWI) MAPK signalling pathway, a signal transduction cascade responsible for the maintenance of cell integrity under adverse environmental conditions. MRNAs whose expression is regulated by this pathway localize in P-bodies after the cell is exposed to stress following a temporal pattern coincident with CWI pathway activation When these mRNAs are overexpressed in a mutant background unable to form visible P-bodies, the cells show hypersensitivity to agents that interfere with cell wall integrity, supporting that they play a role in the mRNA lifecycle under stress conditions. Post-transcriptional control of mRNA is an important mechanism for the regulation of gene expression In this process, the specific localization and compartmentalization of mRNAs within the cytoplasm plays a key role[1]. Our results provide the first evidence that the response to cell wall damage activates a specific transcriptional program, and regulates post-transcriptionally the cell wall-related mRNAs fate
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