Abstract
Summary Microsomal membranes prepared from Daucus carota cell suspension cultures were able to utilize [γ- 32 P]-ATP as a substrate for rapid phosphorylations of endogenous lipids resulting in the labeling of phosphatidic acid, phosphatidylinositol 4-monophosphate, and phosphatidylinositol 4,5-bisphosphate. In the presence of 1μM indoleacetic acid (IAA) the [ 32 P]-label of the phospholipids was significantly reduced within 30s, which was caused by a release of [ 32 P]-labeled inositolphosphates indicating an auxin effect on a phospholipase C-like reaction. Since the kinetics as well as the dose response curves of both IAA-mediated effects were found to correspond well, it is a further confirmation of our previous conclusions that auxin may control a rapid phosphatidylinositol turnover in plant cells, which may be similar to the wellknown phosphoinositide response in animal cells. As discussed, the experimental system presented is certainly a suitable tool for the further analysis of the detailed steps concerning the signal transduction mechanism of auxin on membranes.
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