Signal amplification for simultaneous determination of two proton pump inhibitors in biological matrix based on newly synthesized metal organic framework and polymeric film

Abstract

In this work, by incorporation of newly synthesized metal–organic framework (MOF) into the carbon paste matrix, and electropolymerization of melamine at the resulted electrode, a new sensor was designed for enhanced sensing of omeprazole (OMZ) and pantoprazole (PNZ). Characterization of MOF was accomplished by X-ray diffraction, scanning electron microscopy, Brunauer-Emmett-Teller (BET) surface area analysis, and FT-IR. The influence of affective factors on the electrode quality was inspected. As a result, the carbon paste electrode modified with 7% MOF and applying 30 cycles in polymeric condition wielded notable enhancement for OMZ and PNZ' signals in Britton-Robinson buffer solution (pH 6.0). Three and two linear ranges have been gained for OMZ and PNZ concentration within the various ranges of 0.05–60.0 μmol L−1 and 0.6–50.0 μmol L−1, with detection limits of 0.01 μmol L−1 and 0.15 μmol L−1 respectively. The rightness of the designed sensor for OMZ and PNZ determination in pharmaceutical formulations and human fluid was verified with acceptable results.