Siglec-F is a novel intestinal M cell marker

Nadezhda Gicheva,Matthew S Macauley,Britni M Arlian,James C Paulson,Norihito Kawasaki

doi:10.1016/j.bbrc.2016.08.055

Nadezhda Gicheva, Matthew S Macauley + Show 3 more

Open Access

https://doi.org/10.1016/j.bbrc.2016.08.055

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Abstract

Intestinal microfold (M) cells are epithelial cells primarily present on Peyer's patches (PPs) in the small intestine. The ability of M cells to shuttle antigens into the PP for appropriate immune responses makes M cells a target for next-generation oral vaccine delivery. In this regard, discovery of M cell-specific receptors are of great interest, which could act as molecular tags for targeted delivery of cargo to M cells. Here, using a monoclonal antibody we generated to the Sialic acid-binding immunoglobulin-like lectin F (Siglec-F), we show that Siglec-F is expressed on mouse M cells in the small intestine. Immunohistochemical analysis of the PP tissue sections shows that Siglec-F is expressed on the surface of the M cell membrane exposed to the intestinal lumen. Anti-Siglec-F antibody injected into the mouse small intestine bound to M cells, demonstrating the potential to target M cells via Siglec-F.

Highlights

Intestinal microfold (M) cells are small intestinal epithelial cells localized on thePeyer’s patches (PPs) [1]
Targets investigated to date include α1-2 fucosylated glycans expressed on murine M cells that are recognized by the monoclonal antibody NKM16-2-4 [4], and glycoprotein 2 (GP2), an M cell specific protein that can be targeted with an anti-GP2 [5, 6]
We found that PP M cells did express Siglec-F when compared to an isotype control at levels comparable to eosinophils (CD45+CD11b+CCR3+) (Fig. 2A and B)

Summary

Introduction

Intestinal microfold (M) cells are small intestinal epithelial cells localized on thePeyer’s patches (PPs) [1]. Targets investigated to date include α1-2 fucosylated glycans expressed on murine M cells that are recognized by the monoclonal antibody NKM16-2-4 [4], and glycoprotein 2 (GP2), an M cell specific protein that can be targeted with an anti-GP2 [5, 6]. We generated a monoclonal antibody recognizing Siglec-F to investigate its cell type expression and biological roles in mouse tissues and cells. We found that Siglec-F protein is expressed on the luminal surface of small intestinal M cells.

Results

Conclusion