Abstract
Siderophores are iron chelating substances that are also known to bind metals such as Molybdenum and Lead. The pigment produced by P. fluorescens is a siderophore whose ability to bind to different metal ions was investigated in this study. Pigment production was increased in the presence of Sodium, Potassium, Lead, Molybdenum and Cadmium. Siderophores were detected using CAS assay and catechol - siderophores by Arnow assay. Peak changes and changes in extinction coefficient values are indicators of complex formation. Maximum difference was observed in siderophore free Iron and Iron-siderophore complexes. Some changes were also noted for siderophore- metal complexes for all metals included in this study. Thus siderophore of P. fluorescens shows a tendency to form complexes with almost all metals tested. Key words: pigment; CAS assay; metal ions; complex formation; peak changes; molar extinction coefficient. DOI: 10.4038/cjsbs.v39i2.3001 Cey. J. Sci. (Bio. Sci.) 39 (2): 147-155, 2010
Highlights
MATERIALS AND METHODSIron, one of the most essential microelementsIoslation of Pseudomonas spp was done on for virtually all living cells, is usually abundantKings A and B medium (Deshmukh, 1997) from in the environment, in soils and rhizopheric soil as well as phyllospheres of natural aquifers
Extraction and TLC Pseudomonas fluorescens was grown for 3 days in Barabhaiyya and Rao medium (BR) medium and Nutrient broth which were suitable for intense pigment production
The elute could not be used in subsequent metal chelation studies as the quantity of siderophores containing elute was too little for metal chelation
Summary
MATERIALS AND METHODSIron, one of the most essential microelementsIoslation of Pseudomonas spp was done on for virtually all living cells, is usually abundantKings A and B medium (Deshmukh, 1997) from in the environment, in soils and rhizopheric soil as well as phyllospheres of natural aquifers. Investigates the ability of siderophores to chelate other metal ions as has been reported for iron. Arnow assay The presence of catechol-phenolic type siderophores was detected using Arnow assay in cell free supernatants grown in low as well as high iron Fiss’s minimal media (Arnow, 1937). Extraction and TLC Pseudomonas fluorescens was grown for 3 days in BR medium and Nutrient broth which were suitable for intense pigment production.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
