Abstract
4-Vinylpyridine is a suitable reagent for blocking cysteine functions in proteins. When methionine-containing proteins or pure methionine were reacted with vinylpyridine followed by hydrolysis in 6 n HCl, we observed the loss of methionine and detected a new ninhydrin-positive compound. This compound was isolated and characterized and its structure was determined as S-β-(4-pyridylethyl)- l-homocysteine. In order to prevent the reaction with methionine, the excess of vinylpyridine is removed after blocking the cysteine residues in the proteins.
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