Abstract
Sida rhomboidea. Roxb leaf extract (SRLE) is being used by the populace of North-East India to alleviate symptoms of diabetes and obesity. We have previously reported its hypolipidemic and anti-diabetic properties. In this study, we report the effect of SRLE on (i) in vivo modulation of genes controlling high fat diet (HFD) induced obesity and (ii) in vitro 3T3L1 pre-adipocyte differentiation and leptin release. Supplementation with SRLE significantly prevented HFD induced increment in bodyweight, plasma lipids and leptin, visceral adiposity and adipocyte hypertrophy. Also, SRLE supplementation reduced food intake, down regulated PPARγ2, SREBP1c, FAS and LEP expressions and up-regulated CPT-1 in epididymal adipose tissue compared to obese mice. In vitro adipogenesis of 3T3L1 pre-adipocytes was significantly retarded in the presence of SRLE extract. Also decreased triglyceride accumulation, leptin release and glyceraldehyde-3-Phosphate dehydrogenase activity along with higher glycerol release without significant alteration of viability of 3T3L1 pre-adipocytes, was recorded. Our findings suggest that prevention of HFD induced visceral adiposity is primarily by down regulation of PPARγ2 and leptin gene expression coupled with attenuation of food intake in C57BL/6J mice. SRLE induced prevention of pre-adipocytes differentiation, and leptin release further substantiated these findings and scientifically validates the potential application of SRLE as a therapeutic agent against obesity.
Highlights
Obesity, a fast spreading epidemic, is a major contributor to the global burden of chronic disease and disability
high fat diet (HFD) induced weight gain was significantly controlled by Sida rhomboidea. Roxb leaf extract (SRLE) supplementation of OB mice
There was a significant increment in the feed efficiency ratio in OB mice compared to LEAN mice (0.071 ± 0.004 vs. 0.030 ± 0.002)
Summary
A fast spreading epidemic, is a major contributor to the global burden of chronic disease and disability. More than one billion adults worldwide are overweight and at least, 300 million of them are clinically obese [1]. Such individuals are maximally prone to type-2 diabetes, cardiovascular disease and hypertension in the long run [2,3]. Induction of obesity in humans is either ‘genetic’ of ‘lifestyle’ related. The latter is a complex intermix of sedentary lifestyle and a high calorie diet amounting to nutritional overload [4,5]. Many traditional herbal preparations have been put through a detailed scrutiny to explore their anti-obesity potential and the underlying mechanism of action [7]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
