Short-term stability of genetic structure in populations of the sea anemone Metridium senile

Abstract

Populations of the sea anemone Metridium senile (L.) were sampled from several locations in eastern North America in two series, one collected from 1977–1979 and the other from 1981–1985. Fourteen populations were sampled twice at one- to six-year intervals. Samples were analyzed for temporal differences in genetic composition at both the single locus and multiple locus levels. Overall patterns of geographic variation in allele frequency did not change between series. Regressions describing clines did not differ significantly, and loci not showing clinal variation in the first series remained similar in the second. Analysis of populations sampled twice produced no systematic evidence of change in allele frequency with time for any of four polymorphic loci. Comparisons with computer simulations of repeated sampling of multiple locus genotypes from panmictic populations with free recombination also revealed little temporal change at this level. One population showed possible evidence of recruitment from a different gene pool. Other significant departures from expectations reflected more reproducibility of genotype distributions between samples than expected for sexual populations. This excess stability likely results jointly from clonal reproduction and little sexual recruitment. Despite these indications of genetic stability in adult populations, newly settled juveniles were genetically different from resistant adults in one population, demonstrating the potential for genetic change by immigration. Successful sexual recruitment seems to be rare, even though larvae regularly settle from the plankton. Although interpretation of these results is somewhat limited by lack of knowledge of longevities and generation times, to the extent that they reflect longer-term trends, they suggest that at least some of the observed patterns of geographic variation in allele frequency probably result from natural selection.