Short-term full kindling of the amygdala dissociates natural and periaqueductal gray-evoked flight behaviors of the rat

Abstract

Panic attacks present a high comorbidity with agoraphobia, separation anxiety and generalized anxiety disorder. Nevertheless, while panic attacks have been frequently equated to fear, the relationship of panic disorder with specific phobias remains uncertain. The combination of experimental models of panic and phobias could but afford valuable information about both the comorbidity and causation of these disorders. As it regards, while the defensive behaviors produced by stimulation of dorsal periaqueductal gray matter (DPAG) resemble a panic attack, resistance to capture (RC) behaviors that ensue the kindling of the amygdala (AMY) are reminiscent of a phobic reaction. Therefore, this study examined the thresholds of DPAG-evoked panic-like behaviors in rats showing RC behaviors. Rats bearing electrodes in the DPAG and right AMY were subjected to either the full- (Kin-F) or sham- (Kin-S) kindling of AMY. RC behaviors were evaluated throughout both kindling procedures. Thresholds of DPAG-evoked defensive behaviors were recorded before and after the kindling. Moreover, performances of Kin-F and Kin-S rats in the elevated plus-maze were compared to those of intact controls. Data showed that Kin-F and Kin-S rats perform similarly in the elevated plus-maze, thereby making AMY-kindled rats unlikely as a model of generalized anxiety disorder. On the other hand, whereas the RC is characterized by a marked facilitation of natural freezing and flight behaviors, DPAG-evoked freezing (immobility and exophthalmus) and flight (galloping) behaviors were unexpectedly attenuated. Data suggest that RC is mediated by circuits distinct from those of DPAG-evoked defensive behaviors. Consequently, panic and phobic attacks may be mediated by different mechanisms as well.